He is from Powell River, British Columbia. Pequin developed a love for drawing, and eventually decided to make comics of his own. He attended and graduated high school at Brooks Secondary School.
After college, he would make his own online comic strip, entitled Three Word Phrase; describing the comic, Gerry Mak writes: "Ryan Pequin’s Three Word Phrase web comics are completely absurd, often non-sensical, and completely juvenile like doodles passed around in a high school biology class, which is what makes them hilarious." He has published two volumes worth of Three Word Phrase as books. Pequin also worked on the comic rendition of Bravest Warriors. He was hired to work on Regular Show as a storyboard artist.
He resides in Los Angeles, California, and is in a relationship with a fellow artist, named Emily Partridge.